wnt16 protein  (R&D Systems)

Journal: Calcified Tissue International

Article Title: WNT16 Overexpression is Insufficient to Counteract Inflammation-induced Bone Loss in Female Mice

doi: 10.1007/s00223-026-01481-2

Figure Lengend Snippet: TNF-α reduces Wnt16 mRNA expression in osteoblasts, but WNT16 treatment does not prevent periarticular bone loss in antigen-induced arthritis. A Wnt16 mRNA expression in primary mouse calvarial osteoblasts cultured for 24 h in control medium with or without TNF-α (50 ng/ml). Values are presented as mean ± SEM (n = 4). Data were analyzed using an unpaired Student’s t-test. B Schematic representation of the antigen-induced arthritis model. Upper panel: Control group after secondary immunization (left knee = arthritic; right knee = non-arthritic). Lower panel: Liposome-treated group (left knee = arthritic + liposomes with WNT16; right knee = arthritic + empty liposomes). (C-F) Peripheral quantitative computed tomography (pQCT) analysis of bone mineral density (BMD): total BMD in the metaphyseal regions of the distal femur C and proximal tibia D , and trabecular BMD in the same regions of the distal femur E and proximal tibia F . Data were collected from bones adjacent to non-arthritic and antigen-induced arthritic mouse knees treated with PBS, as well as from bones adjacent to arthritic knees treated with liposomes with WNT16 or empty liposomes. Samples were obtained from 9-week-old female wild-type mice. Values are mean ± SEM (n = 5–9 mice per group). Data assessed by paired Student’s t -test. lipo. = liposomes; inj. = injection; w = with; and w/o = without

Article Snippet: The lipid film was then hydrated with 25 μg WNT16 protein (7790-WN-025/cf; R&D Systems) in 400 μl PBS prewarmed to 32 °C.

Techniques: Expressing, Cell Culture, Control, Liposomes, Computed Tomography, Injection

Journal: Calcified Tissue International

Article Title: WNT16 Overexpression is Insufficient to Counteract Inflammation-induced Bone Loss in Female Mice

doi: 10.1007/s00223-026-01481-2

Figure Lengend Snippet: Overexpression of WNT16 does not protect against PAM2-induced bone loss. A Wnt16 mRNA expression in trabecular-rich vertebral bodies. B Bone volume and C representative 3-dimensional images of calvarial bones analyzed by µCT. D Bone volume over total volume (BV/TV), E trabecular number (Tb. N), and F trabecular thickness (Tb. Th), in femur as measured using µCT. Data were collected from 7-week-old Obl-Wnt16 and wild-type (WT) mice five days after injections of PAM2 or vehicle (control). Values are mean ± SEM (n = 6–13 mice per group). Statistical analyses were performed using a two-way ANOVA. ns = non-significant

Article Snippet: The lipid film was then hydrated with 25 μg WNT16 protein (7790-WN-025/cf; R&D Systems) in 400 μl PBS prewarmed to 32 °C.

Techniques: Over Expression, Expressing, Control

Journal: Calcified Tissue International

Article Title: WNT16 Overexpression is Insufficient to Counteract Inflammation-induced Bone Loss in Female Mice

doi: 10.1007/s00223-026-01481-2

Figure Lengend Snippet: Overexpression of WNT16 does not protect against S . aureus -induced arthritis or bone loss. A Kaplan–Meier survival curve showing the percentage of surviving mice over time following S . aureus inoculation (n = 15 per group). Statistical comparison was performed using the log-rank test. Body weight change B and clinical arthritis scores C over the 8-day period post-inoculation. Data are presented as mean ± SEM (n = 13–15 mice per group) and were analyzed using the Mann–Whitney U test. (D-F) µCT analysis of the fifth lumbar vertebra 8 days post-inoculation, showing D bone volume over total volume (BV/TV), E trabecular number (Tb.N), and F trabecular thickness (Tb.Th) in PBS-treated controls and S. aureus -inoculated mice. Statistical analysis was performed using two-way ANOVA. s.a. = S . aureus ; ns = non-significant

Article Snippet: The lipid film was then hydrated with 25 μg WNT16 protein (7790-WN-025/cf; R&D Systems) in 400 μl PBS prewarmed to 32 °C.

Techniques: Over Expression, Comparison, MANN-WHITNEY

Journal: Scientific Reports

Article Title: Coordination of WNT signaling and ciliogenesis during odontogenesis by piezo type mechanosensitive ion channel component 1

doi: 10.1038/s41598-019-51381-9

Figure Lengend Snippet: Treatment of Yoda1 upregulates expression of WNT16 gene which promotes mineralization of SHED. ( a , b ) Expression of WNT and DACT3 genes. SHED were cultured with or without 5 μM Yoda1 for 24 hrs. Total RNA was prepared from the cells and analyzed by real-time RT-PCR for WNT expression ( a ) and DACT3 expression ( b ). ( c ) Alizarin Red S staining after WNT16 treatment to SHED. After 7 days of culture of SHED in odontogenic induction media in the presence or absence of 300 ng/ml exogenous WNT16, Alizarin Red staining was performed in SHED. The Alizarin Red-positive areas were quantified using ImageJ. Scale bar, 150 μm. The data, as shown, are representative of three independent experiments with similar results, and error bars indicate standard deviations. Statistical analysis was performed using analysis of variance (** p < 0.01).

Article Snippet: Yoda1 and XAV939 were purchased from Tocris Bioscience (Bristol, UK) and recombinant human WNT16 protein from R&D systems (Minneapolis, USA).

Techniques: Expressing, Cell Culture, Quantitative RT-PCR, Staining

Journal: Mucosal immunology

Article Title: Macrophages promote epithelial proliferation following infectious and non-infectious lung injury through a Trefoil factor 2-dependent mechanism

doi: 10.1038/s41385-018-0096-2

Figure Lengend Snippet: ( a,b ) Volcano plots showing results of a cDNA-based screen for Wnt pathway target genes expressed as the fold-difference between ( a ) WT Mφ exposed to damaged or quiescent epithelia and ( b ) between WT Mφ vs. TFF2 −/− Mφ exposed to damaged epithelia. BMMφ were recovered at d4 of MERA for analysis. Each point represents mean of three biological replicates. ( c ) TER values during MERA with WT Mφ in the presence of anti-Wnt4a mAb or control IgG. ( d, e ) Flow-sorted AM, IM and CD103 + DC from WT or TFF2 KO lungs at d4 after infection were analyzed for mRNA levels of ( d ) Wnt4 and ( e ) Wnt16 . ( f ) Representative flow plots and ( g ) quantification of BrdU + SpC + epithelia from distal lung digest cells pre-gated on the live, CD45 − , EpCAM + population for each designated genotype following i.n. administration of rWnt4/16+ R-Spondin 1 cocktail (1μg/mouse, 2 doses) or saline and analyzed by flow cytometry at d4 following N.b. infection (N=5/group). Graphs show Mean±SEM. *, p<0.05; **, p<0.01 and ***, p< 0.005 as determined by ANOVA or Student’s t-test.

Article Snippet: All reagents, including carrier-free recombinant mouse Wnt4, human Wnt16 and mouse R-Spondin 1, were purchased from R&D Systems.

Techniques: Control, Infection, Saline, Flow Cytometry